abstract：:BCG infection of mice provides a convenient model to study natural and cellular immunity to mycobacteria and the mechanisms of granuloma formation and repair. We have used a range of macrophage (M phi) membrane molecules and secretory products to investigate resident M phi-pathogen interactions and T lymphocyte-depend...

journal_title：Immunobiology

authors： Gordon S,Keshav S,Stein M

更新日期：1994-10-01 00:00:00

abstract：:The tubercle bacillus is a facultative intracellular parasite that grows well in non-activated macrophages. When large numbers of these bacilli have grown intracellularly within such macrophages, a cytotoxic immune response, herein called tissue-damaging (or necrotizing) delayed-type hypersensitivity (DTH), kills the ...

journal_title：Immunobiology

authors： Dannenberg AM Jr

更新日期：1994-10-01 00:00:00

abstract：:The history of immunotherapy for tuberculosis is briefly reviewed, and the early appreciation of the importance of secreted antigens, common mycobacterial antigens and stress proteins is noted. The methods by which Mycobacterium vaccae strain NCTC 11659 was selected for special attention, and results of some of the pi...

journal_title：Immunobiology

authors： Stanford JL,Stanford CA

更新日期：1994-10-01 00:00:00

abstract：:Interleukin-6 (IL-6) induced increased, leukocyte and platelet counts on around day 20 when it was administered into [BALB/c-->C3H/He] bone marrow chimeras from day 1 to day 12. Increased leukocyte counts and hemoglobin (Hb) levels were also observed at around day 60 and from day 41 to 80, respectively. On the other h...

journal_title：Immunobiology

authors： Imamura M,Hashino S,Kobayashi H,Tanaka J,Imai K,Han M,Fujii Y,Kobayashi M,Higa T,Kasai M

更新日期：1994-06-01 00:00:00

abstract：:One of the most important effects of aging is the decline of immune effectiveness. A study is described here on the primary (IgM) antibody response to phosphorylcholine (PC), an antigen expressed on the surface of the Streptococcus pneumoniae R36a, in aged (18-22 mo.) C57BL/6 mice. Preliminary data showed a significan...

journal_title：Immunobiology

authors： Nicoletti C

更新日期：1994-02-01 00:00:00

abstract：:Efficient immunoglobulin (Ig) production was induced when human B cells were cultured with autologous T cells activated by immobilized CD3 mAb in cultures supplemented with IL-2. Negatively purified B cells or B cells positively selected with mAb to CD19, CD21 or CD72 surface antigens produced IgM, IgG and IgA, wherea...

journal_title：Immunobiology

authors： Ling NR,Lowe JL

更新日期：1993-12-01 00:00:00

abstract：:Based on our findings that HIV-1 gp41 independently of CD4 can bind to the human helper T lymphoid cell line H9, B cell line Raji and monocyte cell line U937, we characterized putative binding of HIV-1 gp41 to human peripheral blood lymphocytes (PBLs) and monocytes. Using flow cytometry (FACS), we demonstrated that th...

journal_title：Immunobiology

authors： Chen YH,Böck G,Vornhagen R,Steindl F,Katinger H,Dierich MP

更新日期：1993-08-01 00:00:00

abstract：:In the present report, we describe that synthetic inhibitors of and polyclonal and monoclonal antibodies against the membrane ectoenzyme dipeptidyl peptidase IV (DP IV, CD26) inhibit the production of IL-2 and IL-6 and, concomitantly, DNA synthesis of pokeweed mitogen-stimulated peripheral blood mononuclear cells (PBM...

journal_title：Immunobiology

authors： Reinhold D,Bank U,Bühling F,Neubert K,Mattern T,Ulmer AJ,Flad HD,Ansorge S

更新日期：1993-08-01 00:00:00

abstract：:Non-antigen-specific activation of human B lymphocytes for IgE production in vitro requires the presence of interleukin 4 and non-cognate physical interaction with T cells. The latter can be replaced by antibodies directed against the B cells' CD40 structure. Antigen-specific induction of immunoglobulin responses, inc...

journal_title：Immunobiology

authors： Armerding D,van Reijsen FC,Hren A,Mudde GC

更新日期：1993-07-01 00:00:00

abstract：:Mono Mac 6 is a human monocytic cell line with several features of mature blood monocytes such as CD 14 antigen expression, phagocytotic ability, and the functional ability to produce cytokines. This line is often used as an in vitro model to demonstrate the actions of monocytes. In our study, the production of cytoki...

journal_title：Immunobiology

authors： Neustock P,Brand JM,Kruse A,Kirchner H

更新日期：1993-07-01 00:00:00

abstract：:Three different anti-CD26 monoclonal antibodies (mAbs) are described, which specifically inhibited proliferation of human T lymphocytes after stimulation with PHA, tetanus toxoid or soluble anti-CD3 mAb. Anti-CD26 mAbs induced in T cells a dose-dependent shift of the maximum of DNA synthesis, which was due to a transi...

journal_title：Immunobiology

authors： Mattern T,Ansorge S,Flad HD,Ulmer AJ

更新日期：1993-06-01 00:00:00

abstract：:L-alanine was shown earlier to play a significant role for the proliferation of lymphocytes in vitro. In the present work the effect of L-alanine and some other amino acids on thymocyte proliferation was studied in vivo by local administration into one thymus lobe of guinea pigs. Proliferating cells were pulse labelle...

journal_title：Immunobiology

authors： Hägglund B,Sandberg G

更新日期：1993-06-01 00:00:00

abstract：:Cytokines are important mediators of the effects observed after the administration of endotoxin. One of them, tumor necrosis factor, is particularly important since it plays a cardinal role in two major endotoxin activities: its antitumor effect and its capacity to induce a systemic inflammatory response syndrome. TNF...

journal_title：Immunobiology

authors： Brouckaert P,Libert C,Everaerdt B,Takahashi N,Cauwels A,Fiers W

更新日期：1993-04-01 00:00:00

abstract：:We examined the expression of seven V alpha or V beta T cell receptor (TCR) segments on human CD4+ and CD8+ T cells. Confirming previously published results, we found a preferential expression of four V segment gene products on CD4+ T cells. One of these markers (V beta 6.7) was constantly expressed on more CD4+ T cel...

journal_title：Immunobiology

authors： Dannecker G,Mecheri S,Heuser M,Ihle J,Hoffmann MK,Niethammer D

更新日期：1993-01-01 00:00:00

abstract：:Purified CD8+ T cells from influenza A/WSN-immune BALB/c (H-2d) mice respond with the generation of secondary A/WSN-specific Tc cells in vitro when stimulated with a synthetic peptide (NPP) with a sequence derived from influenza A virus nucleoprotein with high affinity for Kd class I MHC molecules. The process of the ...

journal_title：Immunobiology

authors： Kos FJ,Müllbacher A

更新日期：1992-11-01 00:00:00

abstract：:Mycoplasma arthritidis produces a so-far only partially characterized soluble material (MAS) that has a potent mitogenic effect on T lymphocytes of several species. Similar to staphylococcal enterotoxins and a number of related toxins secreted by other species of bacteria, nanogram quantities of these so-called supera...

journal_title：Immunobiology

authors： Brand JM,Kirchner H,Neustock P,Kruse A

更新日期：1992-11-01 00:00:00

abstract：:Hematopoietic cells express multiple receptors which bind the Fc domain of IgG. We utilized transfection of COS-1 cells, a cell line which lacks endogenous Fc receptors, to study the expression and function of Fc gamma RI, the high affinity Fc gamma receptor in the absence of other Fc gamma receptors. Fc gamma RI was ...

journal_title：Immunobiology

authors： Indik Z,Chien P,Levinson AI,Schreiber AD

更新日期：1992-08-01 00:00:00

abstract：:Cytokines have profound effects on cells, and act through receptors which need only be at low concentrations (around 100 copies per cell) to transmit activation signals. The detection of such low concentrations is possible using monoclonal antibodies and fluorescence/flow cytometry, but only by using specialized techn...

journal_title：Immunobiology

authors： Zola H,Flego L,Sheldon A

更新日期：1992-08-01 00:00:00

abstract：:A soluble form of complement receptor number 2 (sCR2) found in human serum closely resembles that produced in culture by B lymphoblastoid cells. Epitope analysis with a panel of CD21 monoclonal antibodies revealed only minor differences between antigen from the two sources. Purified sCR2 from both sources bound to C3d...

journal_title：Immunobiology

authors： Ling NR,Brown B

更新日期：1992-08-01 00:00:00

abstract：:The Fc gamma RIII receptor (CD16) has been described on natural killer cells and a small subset of T lymphocytes. CD16+bright lymphocytes represent the typical population of peripheral blood CD3- NK cells. In these studies in addition to CD16+bright NK cells Fc gamma RIII expressing cytotoxic T lymphocytes in peripher...

journal_title：Immunobiology

authors： Uciechowski P,Werfel T,Leo R,Gessner JE,Schubert J,Schmidt RE

更新日期：1992-06-01 00:00:00

abstract：:We examined the cell cycle distribution and subset marker characteristics of mucosal-associated supramammary lymph node (MALN) T cell subpopulations at sites proximal and distal to the mammary region of animals repeatedly injected with Staphylococcus aureus antigen. Multiparameter flow cytometric analysis of draining ...

journal_title：Immunobiology

authors： Dobrzanski MJ,Yang TJ

更新日期：1992-06-01 00:00:00

abstract：:By quantitative and functional methods, changes were assessed in NK(ASGM-1+) cell numbers and NK cell-mediated lytic function of the spleen and bone marrow of mice bearing a tumor of hemopoietic origin (FLV-induced erythroleukemia) for 9 days +/- simultaneous administration of indomethacin (10 micrograms/ml drinking w...

journal_title：Immunobiology

authors： Christopher FL,Dussault I,Miller SC

更新日期：1991-12-01 00:00:00

abstract：:Activated macrophages have various characteristics in common with exudate and resident macrophages, but the ability to inhibit intracellular proliferation of the protozoa Toxoplasma gondii, the expression of Ia antigen and the capacity to produce H2O2 varies among these cells. Assessment of these features of macrophag...

journal_title：Immunobiology

authors： Nibbering PH,Langermans JA,van de Gevel JS,van der Hulst MB,van Furth R

更新日期：1991-12-01 00:00:00

abstract：:Guinea pig (gp) platelets react to nanomolar doses of the complement-derived anaphylatoxin C5a with a shape change, aggregation and release of biogenic amines and nucleotides from their granules. We have investigated the specific receptor for C5a on gp platelets which mediates these biological effects. Competitive bin...

journal_title：Immunobiology

authors： Kretzschmar T,Kahl K,Rech K,Bautsch W,Köhl J,Bitter-Suermann D

更新日期：1991-11-01 00:00:00

abstract：:We studied immunohistological and biochemical aspects of the CD44 molecule with a mAb produced in our lab: GRHL-1. The characteristic expression of this antigen in cells of B lineage was analyzed. This mAb showed identical immunohistological patterns of reactivity to other mAbs included in CD44 cluster, on a variety l...

journal_title：Immunobiology

authors： Collado A,de Andres A,Cañadas E,Ruiz-Cabello F,Gomez O,Pedrinaci S,Garrido F

更新日期：1991-09-01 00:00:00

abstract：:The distribution of MEL-14+ lymphocytes was investigated by both fluorocytometric analysis and complement-dependent-cellular-cytotoxicity (CDCC) tests in which rabbit anti-rat Ig was added with complement at a secondary step. When CDCC was employed to detect MEL-14+ cells, almost half of the thymocytes were found to b...

journal_title：Immunobiology

authors： Iwabuchi K,Ohgama J,Ogasawara K,Iwabuchi C,Negishi I,Good RA,Onoé K

更新日期：1991-03-01 00:00:00

abstract：:A long-lasting skin tolerance to non-H-2 alloantigens has been reported to be readily induced, when the recipient mice were primed intravenously (i.v.) with 5 x 10(7) spleen cells (SC) plus 1.5 x 10(7) bone marrow cells (BMC) from the H-2 identical strains of mice, and treated intraperitoneally (i.p.) with 200 mg/kg C...

journal_title：Immunobiology

authors： Tomita Y,Nomoto K

更新日期：1990-11-01 00:00:00

abstract：:In contrast to murine spleen M phi, resident peritoneal M phi from health mice express very little Forssman glycolipid antigen (Fo). The following experiments suggest that Fo expression by peritoneal M phi may be associated with inflammation. Balb/c and CBA/J mice were given inflammatory stimuli by i.p. injection of l...

journal_title：Immunobiology

authors： von Kleist R,Schmitt E,Westermann J,Mühlradt PF

更新日期：1990-06-01 00:00:00

abstract：:"Brucelline INRA" is an aqueous extract of a rough strain of Brucella melitensis that is used to test cutaneous delayed hypersensitivity in man and animals. The cellular response of mice (either normal, or sensitized by a previous brucella infection) was investigated with local implanted "cell-traps" and by immunologi...

journal_title：Immunobiology

authors： Bureau JP,Caravano R,Garelli L,Lemaire J

更新日期：1989-03-01 00:00:00

abstract：:This paper describes the characteristics of T suppressor inducer (Ts ind) cells which can interact with T suppressor effector (Ts eff) cells and thereby can account for suppression of delayed-type hypersensitivity (DTH) to alloantigens. Adoptive transfer of spleen cells from mice intravenously (i.v.) injected with all...

journal_title：Immunobiology

authors： Molendijk A,van Gurp RJ,Knulst AC,Benner R

更新日期：1989-03-01 00:00:00

abstract：:We utilized antisera specific for murine IL 1 alpha and IL 1 beta proteins to characterize cell-associated IL 1 according to two distinct criteria, immunochemical detection of radiolabeled IL 1 polypeptides and inhibition of IL 1 activity in cell lysates. Evidence is presented that IL 1 alpha and IL 1 beta are each sy...

journal_title：Immunobiology

authors： Günther C,Röllinghoff M,Beuscher HU

更新日期：1989-02-01 00:00:00

abstract：:The immunoglobulin production capacities of peripheral blood lymphocytes obtained from patients with various chronic inflammatory liver diseases and from normal individuals were compared. Using a reverse hemolytic plaque assay, immunoglobulin-secreting cells (ISC) were counted immediately after isolation (immediate IS...

journal_title：Immunobiology

authors： Sieber G,Teichmann JV,Möller B,Hopf U

更新日期：1988-12-01 00:00:00

abstract：:Kinetic methods can provide significant information concerning the mechanism of cellular cytotoxicity reactions. Previous kinetic studies of cytotoxic T lymphocytes (Tc) have been hampered by the heterogeneity of the effector cell population tested. We therefore examined the kinetics of lysis mediated by cloned, IL 2 ...

journal_title：Immunobiology

authors： Callewaert DM,Meyers P,Hiernaux J,Radcliff G

更新日期：1988-12-01 00:00:00

abstract：:Neutrophilic granulocytes (PMN) are attracted to sites of inflammation by chemotactic factors, the most potent of which are the complement split product C5a, the leukotriene B4 and the bacterial chemotactic factor-related tripeptide formyl-methionyl-leucyl-phenylalanine (FMLP). In addition to inducing directed migrati...

journal_title：Immunobiology

authors： Kownatzki E,Uhrich S,Grüninger G

更新日期：1988-09-01 00:00:00

abstract：:Monoclonal mouse anti-Thy-1.2 antibody of IgG3 isotype (MTA) inhibits in a significant and long-term manner the regional graft-versus-host reaction (GVHR) when administered to donors as well as to recipients. The process of sensitization in the reaction of delayed type hypersensitivity to SRBC (DTH) is, on the other h...

journal_title：Immunobiology

authors： Sůla K,Sobotková E,Svobodová M,Nouza K

更新日期：1988-07-01 00:00:00

abstract：:We investigated the effect of Lewis Lung carcinoma cells on the production of C3 by murine macrophages and examined the capacity of secreted C3 to opsonize Lewis Lung carcinoma cells. C3 released in culture from macrophages obtained from tumor-bearing C57Bl/6 mice as well as from normal macrophages exposed to Lewis Lu...

journal_title：Immunobiology

authors： Lipari M,Di Renzo L,Zicari A,Schulz TF,Magliocca A,Mardente S,Dierich MP,Pontieri GM

更新日期：1988-07-01 00:00:00

abstract：:Chromosome 1 of the mouse carries a number of genes for alloantigens which can be recognized by T cells, among those the mixed lymphocyte stimulation (Mls) locus and the lymphocyte stimulating determinant (Lsd) locus have been described. The Relationship between the antigens coded for by these two loci has been furthe...

journal_title：Immunobiology

authors： Goldbach J,Prahl H,Stäb F,Steinhausen D,Kölsch E

更新日期：1988-05-01 00:00:00

abstract：:The supernatant from cultures of T cell clones derived from (BALB/c----C3H/He) chimeras suppresses BALB/c anti-C3H/He or BALB/c anti-C57BL/6 MLRs. When we studied the alloantigen specificity of the suppressor activity in culture supernatant, we observed three types of the suppressor activity (i.e., the suppressor acti...

journal_title：Immunobiology

authors： Imamura M,Fujimoto H,Fukuhara T,Kobayashi M,Hashino S,Kasai M,Sakurada K,Miyazaki T

更新日期：1988-04-01 00:00:00

abstract：:Neopterin is produced in large amounts specifically from macrophages upon stimulation with interferon-gamma (IFN-gamma). Measurement of neopterin allows a direct in vivo quantification of T cell activation. This is particularly useful, e.g., in early diagnosis of graft rejection. Since disease states with elevated neo...

journal_title：Immunobiology

authors： Fuchs D,Ebell W,Friedrich W,Hausen A,Reibnegger G,Werner ER,Wachter H

更新日期：1988-04-01 00:00:00

abstract：:Splenic B cells of BALB/c mice were stimulated in vitro either with lipopolysaccharide (LPS), dextran sulphate (DxS), or with both LPS and DxS. The absolute frequency of B cells that differentiate into clones of immunoglobulin (Ig)-secreting cells upon activation with these mitogens was determined by means of limiting...

journal_title：Immunobiology

authors： Bos NA,Meeuwsen CG,de Visser H,Benner R

更新日期：1988-02-01 00:00:00